Plasmid_Backbone
Part:BBa_K1137005
Designed by: Matthew Deyell Group: iGEM13_Paris_Bettencourt (2013-10-04)
pSB10K1 – contains mRFP cassette (J04450) and additionally terminators and flanking region from pSB1C3. The origin of replication has been replaced with ColA replicon with a copy number of 20 – 40 per cell. The cat gene has been replaced with kan cassette conveying kanamycin resistance.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Illegal suffix found at 1091
Illegal XbaI site found at 15 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal NheI site found at 1918
Illegal SpeI site found at 1092
Illegal PstI site found at 1106
Illegal NotI site found at 6
Illegal NotI site found at 1099 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Illegal suffix found at 1092
Illegal XbaI site found at 15 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 15
Illegal SpeI site found at 1092
Illegal PstI site found at 1106
Illegal AgeI site found at 802
Illegal AgeI site found at 914
Illegal AgeI site found at 3006 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2933
[edit]
Categories
Parameters
None |