Plasmid_Backbone

Part:BBa_K1137005

Designed by: Matthew Deyell   Group: iGEM13_Paris_Bettencourt   (2013-10-04)

pSB10K1 – contains mRFP cassette (J04450) and additionally terminators and flanking region from pSB1C3. The origin of replication has been replaced with ColA replicon with a copy number of 20 – 40 per cell. The cat gene has been replaced with kan cassette conveying kanamycin resistance.

PB_PSB10K1.png

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Illegal suffix found at 1091
    Illegal XbaI site found at 15
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NheI site found at 1918
    Illegal SpeI site found at 1092
    Illegal PstI site found at 1106
    Illegal NotI site found at 6
    Illegal NotI site found at 1099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Illegal suffix found at 1092
    Illegal XbaI site found at 15
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 15
    Illegal SpeI site found at 1092
    Illegal PstI site found at 1106
    Illegal AgeI site found at 802
    Illegal AgeI site found at 914
    Illegal AgeI site found at 3006
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2933


[edit]
Categories
Parameters
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